KMID : 0381120100320060583
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Genes and Genomics 2010 Volume.32 No. 6 p.583 ~ p.591
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Molecular cloning of GnRH1 gene and GTH cDNAs of the protogynous longtooth grouper, Epinephelus bruneus
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Hyeon Kyeong-Man
Jeong Hyung-Bok Lim Bong-Soo Hur Sung-Pyo Lee Young-Don Park Ji-Gweon Kim Se-Jae
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Abstract
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In teleosts, gonadotropin-releasing hormone (GnRH) and gonadotropin hormone (GTH) play important roles in regulating gonadal development and maturation. In Southeast Asia, the longtooth grouper, Epinephelus bruneus, is a commercially important aquaculture fish. In this study, we cloned and characterized the longtooth grouper GnRH1 gene and cDNAs of three gonadotropin subunits (GTH¥á, FSH¥â, LH¥â). The GnRH1 gene of longtooth grouper was 4, 032 bp long, and contained four exons, 61, 151, 99, and 423 bp long. GTH¥á, FSH¥â, and LH¥â cDNAs were 509, 576, and 579 bp, respectively. Phylogenetic and Southern hybridization analyses revealed that the longtooth grouper GTH subunits were evolutionarily close to those of groupers and are one-copy genes. RT-PCR analyses showed that GTH subunit mRNAs were expressed at a higher level in the pituitary glands of immature fish than in those of mature fish, suggesting a role in gonadal maturation.
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KEYWORD
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Epinephelus bruneus, Gonadotropin hormone (GTH), Gonadotropin-releasing hormone (GnRH), Longtooth grouper
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